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| http://dx.doi.org/10.4161/hv.29044 | DOI Listing |
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Mouse Intestinal Organoid Culture Protocol.
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Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, İstanbul, Turkey.
The discovery of leucine-rich-containing G-protein-coupled receptor 5 (Lgr5) as an intestinal adult stem cell marker had blazed a trail in stem cell biology and laid the foundations of modern organoid technology. Up to date, several well-established intestinal organoid protocols have been reported in the literature from different sources, including adult and induced pluripotent stem cells. Here, we demonstrate a BALB/c mouse-derived intestinal organoid culture protocol, passaging, and cryopreservation procedures.
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Programme de Bioéthique, École de santé publique, Université de Montréal, Montréal, Canada.
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HCA Healthcare Clinical Services Group, Nashville, TN.
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