Objective: To test the feasibility of DNA barcoding for accurate identification of Jinqian Baihua She and its adulterants.
Materials And Methods: Standard cytochrome C oxidase subunit I (COI) gene fragments were sequenced for DNA barcoding of 39 samples from 9 snake species, including Bungarus multicinctus, the officially recognized origin animal by Chinese Pharmacopoeia, and other 8 adulterate species. The aligned sequences, 658 base pairs in length, were analyzed for divergence using the Kimura-2-parameter (K2P) distance model with MEGA5.0.
Results: The mean intraspecific K2P distance was 0.0103 and the average interspecific genetic distance was 0.2178 in B. multicinctus, far greater than the minimal interspecific genetic distance of 0.027 recommended for species identification. A neighbor-joining (NJ) tree was constructed, in which each species formed a monophyletic clade with bootstrap supports of 100%. All the data were submitted to Barcode of Life Data system version 3.0 (BOLD, http://www.barcodinglife.org) under the project title "DNA barcoding Bungarus multicinctus and its adulterants". Ten samples of commercially available crude drugs of JBS were identified using the identification engine provided by BOLD. All the samples were clearly identified at the species level, among which five were found to be the adulterants and identified as Dinodon rufozonatum.
Conclusion: DNA barcoding using the standard COI gene fragments provides an effective and accurate means for JBS identification and authentication.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239722 | PMC |
| http://dx.doi.org/10.4103/0973-1296.141816 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unexpected species diversity in the understanding of selenium-containing soil invertebrates.
Sci Rep
January 2025
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, College of Biology and Agricultural Resources, Hubei Zhongke Research Institute of Industrial Technology, Huanggang Normal University, Huanggang, 438000, Hubei, China.
Yutangba, situated in Enshi City, Hubei Province, is globally noted for its high selenium (Se) content. Soil invertebrates are essential to the functionality and services of terrestrial ecosystems, yet their community composition in this region remains under-explored. This study utilized environmental DNA metabarcoding to investigate the interrelations among environmental factors, soil invertebrate diversity, and community characteristics concerning soil Se content, pH, and moisture content in the region.
View Article and Find Full Text PDFsp. nov., the first representative of the New World micromoth genus Busck (Lepidoptera, Gelechiidae) associated with a member of Asteraceae.
Biodivers Data J
January 2025
Universidad de Tarapacá, Facultad de Ciencias Agronómicas, Departamento de Recursos Ambientales, Arica, Chile Universidad de Tarapacá, Facultad de Ciencias Agronómicas, Departamento de Recursos Ambientales Arica Chile.
Background: The New World micromoth genus Busck, 1939 (Lepidoptera, Gelechiidae, Gelechiinae, Gnorimoschemini) includes 21 described species, ten of which occur in South America. Like the tomato pinworm, (Walsingham, 1897), all the species of , whose host plants have been documented, are associated exclusively with members of the family Solanaceae.
New Information: sp.
A faunal inventory of methane seeps on the Pacific margin of Costa Rica.
Zookeys
January 2025
Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, USA University of California San Diego La Jolla United States of America.
The methane seeps on the Pacific margin of Costa Rica support extensive animal diversity and offer insights into deep-sea biogeography. During five expeditions between 2009 and 2019, we conducted intensive faunal sampling via 63 submersible dives to 11 localities at depths of 300-3600 m. Based on these expeditions and published literature, we compiled voucher specimens, images, and 274 newly published DNA sequences to present a taxonomic inventory of macrofaunal and megafaunal diversity with a focus on invertebrates.
View Article and Find Full Text PDFMorphological and DNA analysis of pollen grains on butterfly individuals reveal their flower visitation history.
Naturwissenschaften
January 2025
Research Center for Integrative Evolutionary Science, The Graduate University for Advanced Studies, SOKENDAI, Hayama, Japan.
Many butterfly species are conspicuous flower visitors. However, understanding their flower visitation patterns in natural habitats remains challenging due to the difficulty of tracking individual butterflies. Therefore, we aimed at establishing a protocol to solve the problem using the Common five-ring butterfly, Ypthima argus (Nymphalidae: Satyrinae).
View Article and Find Full Text PDFFiddler Crabs (Crustacea: Decapoda: Ocypodidae) From Coastal Ecuador and the Galápagos Islands: Species Descriptions and DNA Barcodes.
Ecol Evol
January 2025
Universidad Regional Amazónica Ikiam Tena Ecuador.
Neotropical regions near the equator are recognized as speciation "hot spots" reflecting their abundant biodiversity. In western South America, the coasts of Panama, Colombia, Ecuador, the Galápagos Archipelago, and northern Peru form the Tropical Eastern Pacific biome. This area has the greatest heterogeneity of sympatric fiddler crab species of any portion of the planet.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!