AI Article Synopsis
- - SMC-4 is an ATPase crucial for chromosome assembly and segregation, but its link to colorectal cancer is not well understood.
- - Using quantitative PCR and Western blot analysis, researchers found that SMC-4 expression is significantly higher in colorectal cancer tissues compared to normal tissues and is associated with various cancer stages and differentiation.
- - The study suggests that reducing SMC-4 levels decreases cancer cell proliferation, indicating it may play a role in colorectal cancer progression and serve as a potential target for therapy.
Article Abstract
Background: Structural maintenance of chromosomes 4 (SMC-4) is a chromosomal ATPase which plays an important role in regulate chromosome assembly and segregation. However, the role of SMC-4 in the incidence of malignancies, especially colorectal cancer is still poorly understood.
Materials And Methods: We here used quantitative PCR and Western blot analysis to examine SMC-4 mRNA and protein levels in primary colorectal cancer and paired normal colonic mucosa. SMC-4 clinicopathological significance was assessed by immunohistochemical staining in a tissue microarray (TMA) in which 118 cases of primary colorectal cancer were paired with noncancerous tissue. The biological function of SMC-4 knockdown was measured by CCK8 and plate colony formation assays. Fluorescence detection has been used to detect cell cycling and apoptosis.
Results: SMC-4 expression was significantly higher in colorectal cancer and associated with T stage, N stage, AJCC stage and differentiation. Knockdown of SMC-4 expression significantly suppressed the proliferation of cancer cells and degraded its malignant degree.
Conclusions: Our clinical and experimental data suggest that SMC-4 may contribute to the progression of colorectal carcinogenesis. Our study provides a new therapeutic target for colorectal cancer treatment.
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| http://dx.doi.org/10.7314/apjcp.2014.15.21.9459 | DOI Listing |
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