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Structure of a designed protein cage that self-assembles into a highly porous cube. | LitMetric

Structure of a designed protein cage that self-assembles into a highly porous cube.

Nat Chem

1] UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, California 90095, USA [2] Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569 USA [3] California Nanosystems Institute, University of California, Los Angeles, California 90095, USA.

Published: December 2014

AI Article Synopsis

  • - Natural proteins offer a flexible approach for building complex structures, but designing them with precise shapes and chemical properties is difficult.
  • - Researchers created a new protein that assembles into a large, cube-shaped cage with specific dimensions and openings, demonstrating effective design capabilities.
  • - The study confirms that it's possible to accurately design large porous assemblies, but achieving even more specific shapes may need to reduce the flexibility of these proteins.

Article Abstract

Natural proteins can be versatile building blocks for multimeric, self-assembling structures. Yet, creating protein-based assemblies with specific geometries and chemical properties remains challenging. Highly porous materials represent particularly interesting targets for designed assembly. Here, we utilize a strategy of fusing two natural protein oligomers using a continuous alpha-helical linker to design a novel protein that self assembles into a 750 kDa, 225 Å diameter, cube-shaped cage with large openings into a 130 Å diameter inner cavity. A crystal structure of the cage showed atomic-level agreement with the designed model, while electron microscopy, native mass spectrometry and small angle X-ray scattering revealed alternative assembly forms in solution. These studies show that accurate design of large porous assemblies with specific shapes is feasible, while further specificity improvements will probably require limiting flexibility to select against alternative forms. These results provide a foundation for the design of advanced materials with applications in bionanotechnology, nanomedicine and material sciences.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239666PMC
http://dx.doi.org/10.1038/nchem.2107DOI Listing

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