The respiratory DMSO reductase from Rhodobacter capsulatus catalyzes the reduction of dimethyl sulfoxide to dimethyl sulfide. Herein, we have utilized this Mo enzyme as an enantioselective catalyst to generate optically pure sulfoxides (methyl p-tolyl sulfoxide, methyl phenyl sulfoxide and phenyl vinyl sulfoxide) from racemic starting materials. A hexaaminecobalt coordination compound in its divalent oxidation state was employed as the mediator of electron transfer between the working electrode and DMSO reductase to continually reactivate the enzyme after turnover. In all cases, chiral HPLC analysis of the reaction mixture revealed that the S-sulfoxide was reduced more rapidly leading to enrichment or isolation of the R isomer.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00775-014-1215-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Purification and Electron Transfer from Soluble c-Type Cytochrome TorC to TorA for Trimethylamine N-Oxide Reduction.
Int J Mol Sci
December 2024
Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, 14476 Potsdam, Germany.
The enterobacterium present in the human gut can reduce trimethylamine N-oxide (TMAO) to trimethylamine during anaerobic respiration. The TMAO reductase TorA is a monomeric, bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor-containing enzyme that belongs to the dimethyl sulfoxide reductase family of molybdoenzymes. TorA is anchored to the membrane via TorC, a pentahemic -type cytochrome which receives the electrons from the menaquinol pool.
View Article and Find Full Text PDFNovel Photoelectron-Assisted Microbial Reduction of Arsenate Driven by Photosensitive Dissolved Organic Matter in Mine Stream Sediments.
Environ Sci Technol
December 2024
College of Environmental Science and Engineering, Hunan University, Changsha 410012, P. R. China.
The microbial reduction of arsenate (As(V)) significantly contributes to arsenic migration in mine stream sediment, primarily driven by heterotrophic microorganisms using dissolved organic matter (DOM) as a carbon source. This study reveals a novel reduction pathway in sediments that photosensitive DOM generates photoelectrons to stimulate diverse nonphototrophic microorganisms to reduce As(V). This microbial photoelectrophic As(V) reduction (PEAsR) was investigated using microcosm incubation, which showed the transfer of photoelectrons from DOM to indigenous sediment microorganisms, thereby leading to a 50% higher microbial reduction rate of As(V).
View Article and Find Full Text PDFDual-directional regulation of extracellular respiration in Shewanella oneidensis for intelligently treating multi-nuclide contamination.
J Hazard Mater
December 2024
Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, China. Electronic address:
Article Synopsis
- Radionuclide contamination is a global issue due to the toxicity and mobility of certain isotopes, and bioreduction using electrochemically active bacteria (EAB) offers a promising solution for nuclear waste treatment.
- A novel Esa quorum sensing (EQS) system was developed to enhance gene expression and optimize the EET pathway in the bacteria Shewanella oneidensis MR-1, allowing for dynamic switching between electron transfer processes based on cell density.
- The engineered bacteria demonstrated significant improvements in reducing iodine (IO) and uranium (U(VI)), with increases of 2.51 and 2.05 times respectively, and a mobile biofilm was created to efficiently collect uranium precipitates, showcasing the EQS system's potential for
The operon encodes an essential and modular electron transfer pathway for extracellular iodate reduction by MR-1.
Microbiol Spectr
August 2024
Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geosciences, Wuhan, Hubei, China.
Extracellular iodate reduction by spp. contributes to iodide generation in the biogeochemical cycling of iodine. However, there is a disagreement on whether spp.
View Article and Find Full Text PDFIdentification of bacterial dissimilatory antimonate reductase AnrA: genes and proteins involved in antimonate respiration and resistance in sp. strain SVR.
Appl Environ Microbiol
March 2024
Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.
sp. strain SVR uses antimonate [Sb(V)] as a terminal electron acceptor for anaerobic respiration. Here, we visualized a possible key enzyme, periplasmic Sb(V) reductase (Anr), via active staining and non-denaturing gel electrophoresis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!