The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.
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| http://dx.doi.org/10.1093/nar/gku1147 | DOI Listing |
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Article Synopsis
- Gcn2 is a protein that helps cells respond when there aren't enough amino acids, which are the building blocks of proteins.
- It stops making normal proteins and instead helps make a special protein that boosts the creation of amino acids.
- Scientists discovered that Gcn2 uses parts similar to an enzyme that normally works with tRNA, but now it helps Gcn2 work in different ways during amino acid shortages.
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