Though portable accelerometers are ubiquitous in physiology and public health studies, their accuracy as objective measures of physical activity is still being examined. This paper enumerates and analyzes the various biases of the widely used ActiLife(®) software in reporting activity counts from ActiGraph(®) accelerometers. In particular, we focus on the two-stage proprietary filtration algorithm used to convert raw acceleration data, for a sampling rate of 30 Hz, to compressed 1 Hz signals; we develop simple novel methods to analyze the action of the software filter on the raw data in the frequency domain.
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