AI Article Synopsis
- c-Myc and phosphatidylinositol 3-OH kinase (PI3K) play key roles in cell cycle regulation and maintaining embryonic stem cell (ESC) characteristics, but their specific relationship in ESCs is not fully understood.
- Research shows that c-Myc and PI3K work together yet separately to help sustain the self-renewal of murine ESCs in standard culture conditions.
- When ESCs are cultured in a specific 2i-condition, which includes inhibitors for GSK3β and MEK, the need for c-Myc and PI3K signaling for maintaining pluripotency decreases, indicating that their roles are context-dependent.
Article Abstract
c-Myc and phosphatidylinositol 3-OH kinase (PI3K) both participate in diverse cellular processes, including cell cycle control and tumorigenic transformation. They also contribute to preserving embryonic stem cell (ESC) characteristics. However, in spite of the vast knowledge, the molecular relationship between c-Myc and PI3K in ESCs is not known. Herein, we demonstrate that c-Myc and PI3K function cooperatively but independently to support ESC self-renewal when murine ESCs are cultured under conventional culture condition. Interestingly, culture of ESCs in 2i-condition including a GSK3β and MEK inhibitor renders both PI3K and Myc signaling dispensable for the maintenance of pluripotent properties. These results suggest that the requirement for an oncogenic proliferation-dependent mechanism sustained by Myc and PI3K is context dependent and that the 2i-condition liberates ESCs from the dependence of this mechanism.
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| http://dx.doi.org/10.1002/stem.1893 | DOI Listing |
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