AI Article Synopsis
- Urinary exosomes are tiny vesicles (<100 nm) released into urine by renal epithelial cells, reflecting the proteins and RNA of their source cells.
- They hold promise as non-invasive biomarkers for kidney diseases due to their unique molecular composition.
- The text outlines a reliable method for preparing urinary exosomes and includes protocols for their biochemical analysis and quantification for comparative proteomic research.
Article Abstract
Urinary exosomes are small (<100 nm) vesicles secreted into urine from renal epithelial cells. They are coated with lipid bilayer, they contain an array of membrane and cytosolic proteins, and selected RNA species, reflecting the molecular composition of their cell of origin. Thus, urinary exosomes have received considerable attention as potential biomarker source, as their proteomic analysis could lead to the discovery of new non-invasive site-specific biomarkers for renal diseases. Here, we describe a robust method for urinary exosome preparation, additional protocols for their biochemical characterization and for the quantitation of different preparations, to be used for comparative proteomic studies.
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| http://dx.doi.org/10.1007/978-1-4939-1872-0_3 | DOI Listing |
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