AI Article Synopsis
- The Mediator complex is essential for transcription by RNA polymerase II and has 15 subunits that work together.
- Researchers successfully recreated and tested this complex, finding that it requires the MED14 subunit to function properly.
- MED14 not only helps stabilize the structure but also improves the complex's interaction with RNA polymerase II, highlighting its key role as both a structural and functional component.
Article Abstract
The evolutionarily conserved Mediator complex is a critical coactivator for RNA polymerase II (Pol II)-mediated transcription. Here we report the reconstitution of a functional 15-subunit human core Mediator complex and its characterization by functional assays and chemical cross-linking coupled to MS (CX-MS). Whereas the reconstituted head and middle modules can stably associate, basal and coactivator functions are acquired only after incorporation of MED14 into the bimodular complex. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. Altogether, our analyses identify MED14 as both an architectural and a functional backbone of the Mediator complex. We further establish a conditional requirement for metazoan-specific MED26 that becomes evident in the presence of heterologous nuclear factors. This general approach paves the way for systematic dissection of the multiple layers of functionality associated with the Mediator complex.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259101 | PMC |
| http://dx.doi.org/10.1038/nsmb.2914 | DOI Listing |
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