AI Article Synopsis
- A new method using photoaffinity labeling (PAL) allows for quick identification of target proteins by attaching a special fluorescent tag (IsoFT) through irradiation.
- Labeled peptides can be easily identified using advanced fluorescence spectroscopy and mass spectrometry, leading to detailed amino acid sequencing.
- This approach reduces both the time and amount of protein needed for identification and is applicable for analyzing proteins in complex mixtures like cell lysates.
Article Abstract
A photoaffinity labeling (PAL)-based method for the rapid identification of target proteins is presented in which a high-performance chemical tag, an isotope-coded fluorescent tag (IsoFT), can be attached to the interacting site by irradiation. Labeled peptides can be easily distinguished among numerous proteolytic digests by sequential detection with highly sensitive fluorescence spectroscopy and mass spectrometry. Subsequent MS/MS analysis provides amino acid sequence information with a higher depth of coverage. The combination of PAL and heterogeneous target-selecting techniques significantly reduces the amount of time and protein required for identification. An additional photocleavable moiety successfully accelerated proteomic analysis using cell lysate. This method is a widely applicable approach for the rapid and accurate identification of interacting proteins.
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| http://dx.doi.org/10.1002/anie.201408580 | DOI Listing |
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