Inter-simple sequence repeat PCR (ISSR-PCR) is a fast, inexpensive genotyping technique based on length variation in the regions between microsatellites. The method requires no species-specific prior knowledge of microsatellite location or composition. Very small amounts of DNA are required, making this method ideal for organisms of conservation concern, or where the quantity of DNA is extremely limited due to organism size. ISSR-PCR can be highly reproducible but requires careful attention to detail. Optimization of DNA extraction, fragment amplification, and normalization of fragment peak heights during fluorescent detection are critical steps to minimizing the downstream time spent verifying and scoring the data.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-1966-6_5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A mono-phasic protocol for micropropagation of potato cv. Cooch Behar local, its acclimatization, on-field evaluation, and fidelity analysis.
3 Biotech
February 2025
Crop Research Unit (Genetics and Plant Breeding), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252 India.
A protocol for micropropagation of potato ( L.) cv. Cooch Behar local retaining the fidelity of the in vitro regenerants was established for the first time.
View Article and Find Full Text PDFPhenotypic simulation for fruit-related traits in F progenies of chili peppers (Capsicum annuum) using genomic prediction based solely on parental information.
Mol Genet Genomics
January 2025
Institute of Agriculture, Academic Assembly Faculty, Shinshu University, 8304 Minamiminowa, Nagano, 399-4598, Japan.
Chili pepper (Capsicum spp.) fruits are used as vegetables, spices, and ornamental plants, necessitating various fruit characteristics. However, their genetic improvement is challenging through conventional crossbreeding due to the quantitative traits, which makes it difficult to predict phenotypes in the progeny.
View Article and Find Full Text PDFDetermination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers.
Sci Rep
January 2025
Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská, 1665/1, 61300, Brno, Czech Republic.
Background: Persimmon (Diospyros kaki L.) belongs to the Ebenaceae family, which includes six genera and about 400 species. This study evaluated the genetic diversity of 100 persimmon accessions from Hatay province, Türkiye using 42 morphological and pomological traits, along with inter simple sequence repeat (ISSR) markers and multivariate analysis.
View Article and Find Full Text PDF<b>Background and Objective:</b> Black soybeans [<i>Glycine max</i> (L.) Merr] are among the important crops, but the cultivated resources are normally low-yielding, susceptible to diseases and low profit. Therefore, it is necessary to evaluate the genetic diversity of black soybean germplasms for breeding programs.
View Article and Find Full Text PDFElucidation of genetic diversity in hawthorn (Crataegus azarolus L.) accessions naturally growing in the Eastern Mediterranean region of Türkiye using morphological, pomological, and inter simple sequence repeat markers.
BMC Plant Biol
January 2025
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.
Background: Türkiye hosts many important fruit species due to its geographical location and ecology. Hawthorn, which is highly beneficial for human health, is one of these significant fruit species. In the present study, 125 accessions of Crataegus azarolus L.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!