AI Article Synopsis
- MHC multimers are powerful tools for directly detecting and analyzing specific T-cell populations, aiding in the study of immune responses in both humans and animals.
- This technology allows for the precise enumeration of T-cells that target viral, tumor, and vaccine antigens with high sensitivity and specificity.
- In HIV/SIV research, MHC multimers provide essential insights into the abundance of HIV/SIV-specific CD8(+) T-cells across different tissues, contributing to our understanding of AIDS progression, vaccine immunogenicity, and T-cell memory formation.
Article Abstract
The use of MHC multimers allows precise and direct detecting and analyzing of antigen-specific T-cell populations and provides new opportunities to characterize T-cell responses in humans and animals. MHC-multimers enable us to enumerate specific T-cells targeting to viral, tumor and vaccine antigens with exceptional sensitivity and specificity. In the field of HIV/SIV immunology, this technique provides valuable information about the frequencies of HIV- and SIV-specific CD8(+) cytotoxic T lymphocytes (CTLs) in different tissues and sites of infection, AIDS progression, and pathogenesis. Peptide-MHC multimer technology remains a very sensitive tool in detecting virus-specific T -cells for evaluation of the immunogenicity of vaccines against HIV-1 in preclinical trials. Moreover, it helps to understand how immune responses are formed following vaccination in the dynamics from priming point until T-cell memory is matured. Here we review a diversity of peptide-MHC class I multimer applications for fundamental immunological studies in different aspects of HIV/SIV infection and vaccine development.
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| http://dx.doi.org/10.1586/14760584.2015.962520 | DOI Listing |
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