A salmonella genomic island, designated SGI11, was found in 18 of 26 multidrug-resistant Salmonella enterica serovar Typhi isolates from Bangladesh. SGI11 was an IS1 composite transposon and carried 7 resistance genes that conferred resistance to 5 first-line antimicrobials. Eleven of the 18 SGI11-carrying S. Typhi isolates had developed resistance to high levels of ciprofloxacin.
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http://dx.doi.org/10.1128/AAC.04081-14 | DOI Listing |
Nucleic Acids Res
January 2025
School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.
Genome-wide identification of binding profiles for DNA-binding proteins from the limited number of intracellular pathogens in infection studies is crucial for understanding virulence and cellular processes but remains challenging, as the current ChIP-exo is designed for high-input bacterial cells (>1010). Here, we developed an optimized ChIP-mini method, a low-input ChIP-exo utilizing a 5,000-fold reduced number of initial bacterial cells and an analysis pipeline, to identify genome-wide binding dynamics of DNA-binding proteins in host-infected pathogens. Applying ChIP-mini to intracellular Salmonella Typhimurium, we identified 642 and 1,837 binding sites of H-NS and RpoD, respectively, elucidating changes in their binding position and binding intensity during infection.
View Article and Find Full Text PDFIntracellular protein production in bacteria is limited by the need for lysis and costly purification. A promising alternative is to engineer the host organism for protein secretion. While the serovar Typhimurium ( .
View Article and Find Full Text PDFJ Anim Physiol Anim Nutr (Berl)
January 2025
Department Animal Science, Higher Education Complex of Torbat-e Jam, Torbat-e Jam, Iran.
This study aimed to compare the effects of dietary supplementation of bacteriophage (BP) and acidifiers on performance, meat quality, morphology, and intestinal microbiota in chickens challenged and unchallenged with Salmonella enteritidis (SE) and also to investigate the possibility of replacing them in the diet with antibiotics. A total of 1760 male Ross (308) chicks were randomly assigned to 11 dietary treatments (8 pens/with 20 male chickens in each). Dietary treatments were as follows: SE-uninfected (negative control (NC), a basal diet without supplemention; NC+ 500 g/t BP (NBP1); NC+ 1000 g/t BP (NBP2); NC+ 300 mg/kg acidifier A (NAA); NC+ 300 mg/kg acidifier B (NAB)) and SE-infected (positive control (PC), a basal diet without supplemention; PC+ 40 mg/kg Antibiotic enrofloxacin (PA); PC+ 500 g/t BP (PBP1); PC+ 1000 g/t BP (PBP2); PC+ 3000 mg/kg acidifier A (PAA); PC+ 3000 mg/kg acidifier B (PAB)).
View Article and Find Full Text PDFProtein Sci
February 2025
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
Polymyxins are last-resort antimicrobial peptides administered clinically against multi-drug resistant bacteria, specifically in the case of Gram-negative species. However, an increasing number of these pathogens employ a defense strategy that involves a relay of enzymes encoded by the pmrE (ugd) loci and the arnBCDTEF operon. The pathway modifies the lipid-A component of the outer membrane (OM) lipopolysaccharide (LPS) by adding a 4-amino-4-deoxy-l-arabinose (L-Ara4N) headgroup, which renders polymyxins ineffective.
View Article and Find Full Text PDFPrev Vet Med
January 2025
Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW 2006, Australia; Sydney Infectious Diseases Institute, The University of Sydney, Camperdown, NSW 2006, Australia. Electronic address:
Pregnancy failure is a serious economic and welfare concern in the Thoroughbred horse industry, yet its incidence and risk factors in Australia remain unclear. This retrospective, nested, case-control study investigated pregnancy failure in resident mares on studs in the Hunter Valley, Australia, in 2021-2022, in early (46-150 days), mid (151-270 days), and late (>270 days) gestation. We found an annual incidence risk of 5.
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