Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228510 | PMC |
| http://dx.doi.org/10.1631/jzus.B1400123 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel Green Crosslinked Salecan Hydrogels and Preliminary Investigation of Their Use in 3D Printing.
Pharmaceutics
January 2023
National Research & Development Institute for Chemistry and Petrochemistry, ICECHIM, Spl. Independentei No. 202, 6th District, 060021 Bucharest, Romania.
Salecan, a kind of polysaccharide, is produced by the Agrobacterium ZX09 salt tolerant strain. In this study, green crosslinked citric acid-salecan hydrogels are explored as novel materials with a high potential for use in regenerative medicine. The impact of salecan and citric acid on the final crosslinked hydrogels was intensively studied and estimated in terms of the whole physicochemical properties and antimicrobial activity.
View Article and Find Full Text PDFsp. ZX09 β-Glucan Attenuates Enterotoxigenic -Induced Disruption of Intestinal Epithelium in Weaned Pigs.
Int J Mol Sci
September 2022
Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
To explore the protective effect of dietary β-glucan (BGL) supplementation on intestinal epithelium exposure to enterotoxigenic Escherichia coli (ETEC), thirty-two weaned pigs were assigned to four groups. Pigs were fed with a basal diet or basal diet containing 500 mg/kg BGL, and were orally infused with ETEC or culture medium. Results showed BGL supplementation had no influence on growth performance in weaned pigs.
View Article and Find Full Text PDFA water-soluble β-glucan improves growth performance by altering gut microbiome and health in weaned pigs.
Anim Nutr
December 2021
Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
Beta-glucan has been shown to have a beneficial effect on gastrointestinal health. This experiment was conducted to investigate the effects of β-glucan isolated from sp. on growth performance and intestinal health of weaning pigs.
View Article and Find Full Text PDFSalecan polysaccharide-based hydrogels and their applications: a review.
J Mater Chem B
April 2019
School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
Polysaccharide-based hydrogels exhibit unique properties like renewability, biocompatibility and biodegradability, thus making them ideal materials for potential utility in tissue engineering scaffolds, drug delivery vehicles, and water purification devices. In the past decade, salecan, a water-soluble β-1,3-glucan secreted by the Agrobacterium sp. ZX09, has become a vital family of polysaccharides for the construction of hydrogels owing to its biofunctionality and susceptibility to chemical modification.
View Article and Find Full Text PDFDietary β-glucan supplementation improves growth performance, carcass traits and meat quality of finishing pigs.
Anim Nutr
December 2019
Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory of Animal Disease Resistance Nutrition, Ministry of Education, Chengdu 611130, China.
This experiment was conducted to investigate growth performance, carcass traits and meat quality of finishing pigs with dietary β-glucan supplementation. A total of 96 healthy pigs (Duroc × Landrace × Yorkshire; initial average BW = 25 kg) were randomly allocated into 4 dietary treatments with 6 replicates per treatment and 4 pigs per replicate. The control group was fed a basal diet, and the experimental diets were supplemented with 50, 100 and 200 mg/kg sp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!