Transcriptional Profiling of a Cross-Protective serovar Typhimurium UK-1 Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments.

Pathogens

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA ; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA ; Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA 24061, USA.

Published: January 2014

AI Article Synopsis

  • Vaccination with a specific strain of Salmonella Typhimurium that lacks DNA adenine methyltransferase can provide immunity against various serotypes, due to changes in gene expression when the bacteria encounter different environments.
  • The research involved comparing the gene activity of this mutant strain with its parent strain in conditions mimicking where it would infect the host, revealing increased transcription of certain protective genes.
  • About 22% of these highly expressed genes were also more active than in other clinically important serovars, suggesting they may play a key role in developing cross-protective immunity against multiple bacteria types.

Article Abstract

Vaccination with serovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multiple serotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. This de-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses. To identify genes encoding proteins that may contribute to cross-protective immunity, we used a Typhimurium DNA adenine methyltransferase mutant strain (UK-1 mutant) derived from the parental UK-1 strain, and assessed the transcriptional profile of the UK-1 mutant and UK-1 strain grown under conditions that simulate the intestinal or endosomal microenvironments encountered during the infective process. As expected, the transcriptional profile of the UK-1 mutant identified a set of genes more transcriptionally active when compared directly to UK-1, and stably transcribed in biologically relevant culture conditions. Further, 22% of these genes were more highly transcribed in comparison to two other clinically-relevant serovars. The strategy employed here helps to identify potentially conserved proteins produced by the UK-1 mutant that stimulate and/or modulate the development of cross-protective immune responses toward multiple serotypes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213855PMC
http://dx.doi.org/10.3390/pathogens3020417DOI Listing

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