Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.

Nat Struct Mol Biol

1] Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA. [2] Molecular Biology Institute, UCLA, Los Angeles, California, USA.

Published: December 2014

Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the β-propeller domain of yeast coronin 1 (crn1), at 8.6-Å resolution. Our structures reveal the main differences in the interaction of coronin with the two nucleotide states of F-actin. We derived pseudoatomic models by fitting the atomic structures of actin and coronin into the EM envelopes and confirmed the identified interfaces on actin by chemical cross-linking, fluorescence spectroscopy and actin mutagenesis. The models offer a structural explanation for the nucleotide-dependent effects of coronin on cofilin-assisted remodeling of F-actin.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388421PMC
http://dx.doi.org/10.1038/nsmb.2907DOI Listing

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