During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software.
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http://dx.doi.org/10.1093/bioinformatics/btu720 | DOI Listing |
Nanomaterials (Basel)
December 2024
Institute of Photonics and Nanotechnology, Faculty of Physics, Vilnius University, Saulėtekio Ave. 3, 10257 Vilnius, Lithuania.
We elaborate a method for determining the 0D-1D nanostructure size by photoluminescence (PL) emission spectrum dependence on the nanostructure dimensions. As observed, the high number of diamond-like carbon nanocones shows a strongly blue-shifted PL spectrum compared to the bulk material, allowing for the calculation of their top dimensions of 2.0 nm.
View Article and Find Full Text PDFBiomimetics (Basel)
December 2024
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Background: The use of ex vivo assays associated with biomaterials may allow the short-term visualization of a specific cell type response inserted in a local microenvironment. Blood is the first component to come into contact with biomaterials, providing blood clot formation, being substantial in new tissue formation. Thus, this research investigated the physiological blood clot (PhC) patterns formed in 3D scaffolds (SCAs), based on chitosan and 20% beta-tricalcium phosphate and its effect on osteogenesis.
View Article and Find Full Text PDFChemistryOpen
December 2024
Department of Forensic Medicine, Nanjing Medical University, Nanjing, 211166, PR China.
The growing demand for detection and sensing in the biomedical field is placing higher demands on technology. In clinical testing, it is expected to be able to realize both rapid large-field imaging and analysis of single particles (or single molecules or single cells), and it is expected to be able to grasp both the unique individuality of single particles in time and space during the complex reaction process, as well as the regular correlation between single particles in the same population distribution. Supported and promoted by the theory of localized surface plasmon resonance (LSPR), dark-field microscopy, as a single-particle optical imaging technique with a very high signal-to-noise ratio, provides a powerful new means to address the above clinical detection needs.
View Article and Find Full Text PDFVirulence
December 2025
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China.
Live herpesvirus-vectored vaccines are critical in veterinary medicine, but they can sometimes offer insufficient protection due to suboptimal antigen expression or localization. Encephalomyocarditis virus (EMCV) is a significant zoonotic threat, with VP1 protein as a key immunogen on its capsid. To enhance immunogenicity, we explored the use of recombinant pseudorabies virus (rPRV) as a vaccine vector against EMCV.
View Article and Find Full Text PDFJ Cachexia Sarcopenia Muscle
February 2025
Mitodicure GmbH, Kriftel, Germany.
Background: Recent studies provide strong evidence for a key role of skeletal muscle pathophysiology in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). In a 2021 review article on the pathophysiology of ME/CFS, we postulated that hypoperfusion and ischemia can result in excessive sodium and calcium overload in skeletal muscles of ME/CFS patients to cause mitochondrial damage. Since then, experimental evidence has been provided that supports this concept.
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