PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy.

Bioinformatics

Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.

Published: March 2015

During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341069PMC
http://dx.doi.org/10.1093/bioinformatics/btu720DOI Listing

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