AI Article Synopsis
- The report outlines the creation of an integrated monitoring system using enzyme-biosensors and chemical sensors to track the metabolic status of mammalian cells.
- A silicon platform was designed using MEMS technology, integrating various sensors (like pH and O2) and biosensors (like glucose and lactate) into a microwell format.
- The system was tested successfully in parallel configurations, demonstrating its potential for applications in cell culture and toxicity research.
Article Abstract
This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279497 | PMC |
| http://dx.doi.org/10.3390/s141120519 | DOI Listing |
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