Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.

Open Anal Chem J

Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8014, USA.

Published: January 2007

This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211269PMC
http://dx.doi.org/10.2174/1874065000701010028DOI Listing

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