AI Article Synopsis
- A disulfide intercalator toolbox was created to allow easy attachment of different functional groups to proteins or peptides in a safe, biological environment.
- Somatostatin (SST), a peptide hormone, was used as a model to test the disulfide functionalization techniques, whether before or after linking it to other molecules.
- A tetrazole-SST version was developed that, when exposed to light, can trigger a specific reaction in live mammalian cells, which researchers observed through imaging.
Article Abstract
A disulfide intercalator toolbox was developed for site-specific attachment of a broad variety of functional groups to proteins or peptides under mild, physiological conditions. The peptide hormone somatostatin (SST) served as model compound for intercalation into the available disulfide functionalization schemes starting from the intercalator or the reactive SST precursor before or after bioconjugation. A tetrazole-SST derivative was obtained that undergoes photoinduced cycloaddition in mammalian cells, which was monitored by live-cell imaging.
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| http://dx.doi.org/10.1002/chem.201403965 | DOI Listing |
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