Reported here is the first example of a ruthenium(iii) complex [Ru(III)(EDTA)(H2O)](-) (EDTA(4-) = ethylenediaminetetraacetate) that mediates S-nitrosylation of cysteine in the presence of nitrite at pH 4.5 (acetate buffer) and results in the formation of [Ru(III)(EDTA)(SNOCy)](-). The kinetics of the reaction was studied by stopped-flow and rapid-scan spectrophotometry as a function of [Cysteine], [NO2(-)] and pH (3.5-8.5). Formation of [Ru(III)(EDTA)(SNOCy)](-), the product of the S-nitrosylation reaction, was identified by ESI-MS experiments. A working mechanism in agreement with the spectroscopic and kinetic data is presented.
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