The study of HIV-infected patients using new five-color panel CytoDiff for flow cytofluorometry revealed the imbalance in subpopulations of leukocytes. The manifestations consisted in significant decrease of total amount of lymphocytes and also CD16+ lymphocytes. B-lymphocytes and eosinocytes with synchronous increase of level of CD16+ monocytes, neutrophils and immature B-lymphocytes. After application to patients of highly active anti-retroviral therapy occurred decrease of viral load under synchronous increase of total level of lymphocytes, CD16+ lymphocytes, B-lymphocytes and also decrease of amount of CD16+ monocytes, neutrophils and immature B-lymphocytes.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Evaluation of the detection of blasts by Sysmex hematology instruments, CellaVision DM96, and manual microscopy using flow cytometry as the confirmatory method.
Int J Lab Hematol
June 2019
Department of multidisciplinary laboratory medicine and medical biochemistry, Akershus University Hospital, Lørenskog, Norway.
Article Synopsis
- The study evaluated how well blast flags from Sysmex instruments (XE/XN) diagnose blasts compared to flow cytometry (IFCM) and other methods like manual microscopy and CellaVision DM96.
- Blood samples were analyzed using various techniques, revealing different levels of sensitivity and specificity for detecting blasts across methods.
- Results indicated that the CellaVision reclassification is almost as effective as manual microscopy for detecting blasts, and the Sysmex XN was better at identifying true positive blasts than the XE.
The study of HIV-infected patients using new five-color panel CytoDiff for flow cytofluorometry revealed the imbalance in subpopulations of leukocytes. The manifestations consisted in significant decrease of total amount of lymphocytes and also CD16+ lymphocytes. B-lymphocytes and eosinocytes with synchronous increase of level of CD16+ monocytes, neutrophils and immature B-lymphocytes.
View Article and Find Full Text PDFThe extended leukocyte differential count using the Cytodiff flow cytometric system reveals that higher CD16+ cytotoxic NK+T lymphocyte levels predict superior survival outcomes in patients with metastatic carcinoma.
Cytometry B Clin Cytom
May 2013
Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, South Korea.
The recently developed Cytodiff flow cytometric system (Beckman Coulter, Miami, FL) enables leukocyte analysis using a single immunophenotyping panel tube composed of six markers and five colors and that can detect 16 leukocyte subpopulations. We performed a preliminary investigation of whether changes in any of 16 leukocyte differentials were associated with survival and treatment outcomes in patients with metastatic carcinoma or not. We measured 16 leukocyte differential counts using the Cytodiff flow cytometric system in peripheral blood samples from 40 patients with metastatic malignancy (27 stomach cancer and 13 lung cancer) before chemotherapy and at 15 day intervals after chemotherapy for 2 months.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!