AI Article Synopsis
- The electrophoretic force on single DNA molecules varies based on the size of the nanocapillary openings and changes along its length.
- Using optical tweezers, researchers measured stalling forces, finding that smaller nanocapillary diameters produce higher forces.
- The study also indicates that for nanocapillaries with openings less than 20 nm, the force profiles mimic those observed in solid-state nanopores, and a combined model was used to analyze these position-dependent forces.
Article Abstract
The electrophoretic force on a single DNA molecule inside a glass nanocapillary depends on the opening size and varies with the distance along the symmetrical axis of the nanocapillary. Using optical tweezers and DNA-coated beads, we measured the stalling forces and mapped the position-dependent force profiles acting on DNA inside nanocapillaries of different sizes. We showed that the stalling force is higher in nanocapillaries of smaller diameters. The position-dependent force profiles strongly depend on the size of the nanocapillary opening, and for openings smaller than 20 nm, the profiles resemble the behavior observed in solid-state nanopores. To characterize the position-dependent force profiles in nanocapillaries of different sizes, we used a model that combines information from both analytical approximations and numerical calculations.
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| http://dx.doi.org/10.1021/nl503272r | DOI Listing |
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