AI Article Synopsis
- Researchers are identifying clonal groups of multidrug-resistant and virulent Klebsiella pneumoniae strains to understand their genetic makeup better.
- They sequenced 48 isolates and compared them to 119 existing genomes, focusing on 694 conserved genes to define hypervirulent and multidrug-resistant groups.
- A new database, BIGSdb-Kp, was created to help access important information about K. pneumoniae genomes, revealing some isolates with both virulence and drug resistance.
Article Abstract
Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely nonoverlapping, isolates with combined virulence and resistance features were detected.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214299 | PMC |
| http://dx.doi.org/10.3201/eid2011.140206 | DOI Listing |
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