In this paper, we improve upon earlier approaches to segmenting mitochondria in Electron Microscopy images by explicitly modeling the double membrane that encloses mitochondria, as well as using features that capture context over an extended neighborhood. We demonstrate that this results in both improved classification accuracy and reduced computational requirements for training.
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Sci Rep
January 2025
Zhongyu (Fujian) Digital Technology Co., Ltd, Fuzhou, 350108, China.
Attention mechanisms have been introduced to exploit deep-level information for image restoration by capturing feature dependencies. However, existing attention mechanisms often have limited perceptual capabilities and are incompatible with low-power devices due to computational resource constraints. Therefore, we propose a feature enhanced cascading attention network (FECAN) that introduces a novel feature enhanced cascading attention (FECA) mechanism, consisting of enhanced shuffle attention (ESA) and multi-scale large separable kernel attention (MLSKA).
View Article and Find Full Text PDFPhys Rev Lett
December 2024
Departamento de Física e Astronomia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal and INESC TEC, Centre of Applied Photonics, Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
Easily accessible through tabletop experiments, paraxial fluids of light are emerging as promising platforms for the simulation and exploration of quantumlike phenomena. In particular, the analogy builds on a formal equivalence between the governing model for a Bose-Einstein condensate under the mean-field approximation and the model of laser propagation inside nonlinear optical media under the paraxial approximation. Yet, the fact that the role of time is played by the propagation distance in the analog system imposes strong bounds on the range of accessible phenomena due to the limited length of the nonlinear medium.
View Article and Find Full Text PDFSmall Methods
January 2025
Division of Molecular and Medical Genetics, Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
Achieving safe and efficient gene therapy hinges upon the inspection of genomes enclosed within individual nano-carriers to mitigate potential health risks associated with empty or fragment-filled vectors. Here solid-state nanopore sensing is reported for identifications of intermediate adeno-associated virus (AAV) vectors in liquid. The method exploits the phenomenon of translocation slowdown induced by the viscosity of salt water-organic mixtures.
View Article and Find Full Text PDFmBio
January 2025
Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Unlabelled: Non-enveloped viruses like poliovirus (PV) have evolved the capacity to spread by non-lytic mechanisms. For PV, this mechanism exploits the host secretory autophagy pathway. Virions are selectively incorporated into autophagosomes, double-membrane vesicles that travel to the plasma membrane, fuse, and release single-membrane vesicles containing virions.
View Article and Find Full Text PDFMar Environ Res
December 2024
University of Coimbra, MARE - Marine and Environmental Sciences Centre / ARNET - Aquatic Research Network, Department of Life Sciences, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal.
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