AI Article Synopsis
- Induced pluripotent stem cells (iPSCs) are created by reprogramming somatic cells using specific embryonic transcription factors, allowing researchers to study diseases and develop treatments.
- This chapter outlines the processes involved in generating iPSCs, including producing MEF-conditioned medium, preparing mitotically arrested mouse embryonic fibroblasts, and using viral vectors for reprogramming.
- While the methods presented are widely used, some cell types may require alternative techniques for effective transduction of reprogramming factors.
Article Abstract
Induced pluripotent stem cells (iPSCs) are generated from somatic cells that have been reprogrammed by the ectopic expression of defined embryonic transcription factors. This technology has provided investigators with a powerful tool for modelling disease and developing treatments for human disorders. This chapter provides the researcher with some background on iPSCs and details on how to produce MEF-conditioned medium, prepare mitotically arrested mouse embryonic fibroblasts (MEFs), create iPSCs using viral vectors, passage iPSCs, and cryopreserve iPSCs. The methods offered here have been used in many laboratories around the world and the reader can initially follow these methods. However, not all cell types are easily transduced using viral vectors and other methods of delivering the reprogramming transcription factors may need to be tested.
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| http://dx.doi.org/10.1007/978-1-4939-1619-1_5 | DOI Listing |
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