Aim: The aim of this study was to make immunohistochemical analyses with Inhibin alpha-subunit, Melan A and MNF116 (pan-Cytokeratin antibody) in pheochromocytomas, because immunohistochemistry is useful for the distinction between adrenal tumors.
Patients And Methods: We used 20 patients with pheochromocytomas submitted to laparoscopic (n=19) or classical (n=1) surgery and we have explored immuno-staining with Inhibin alpha-subunit, Melan A and MNF116 in these tumors. This can be helpful when we cannot make the distinction between adrenal tumors.
Results: Pheochromocytomas did not stain with Inhibin alpha-subunit, Melan A and MNF116.
Conclusions: In our study, Inhibin alpha-subunit, Melan A and MNF116 were not sensitive for pheochromocytomas.
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Int J Mol Sci
September 2024
Pediatric Endocrinology, Diabetology and Metabolism, Department of Pediatrics, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.
Steroidogenic factor 1 (SF-1) is a nuclear receptor that regulates steroidogenesis and reproductive development. /SF-1 variants are associated with a broad spectrum of phenotypes across individuals with disorders of sex development (DSDs). Oligogenic inheritance has been suggested as an explanation.
View Article and Find Full Text PDFHeliyon
September 2024
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832000, China.
Background: Inhibin is a member of the transforming growth factor family that influences reproduction in animals.
Objective: The purpose of this study was to obtain nanobodies from the phage antibody library constructed by us that can specifically bind to inhibin α-subunit.
Methods: In this study, camels were immunized with Kazakh sheep inhibin-α protein that expressed in BL21 , and the camel VHH nanobody phage display library was prepared using nested PCR.
Medicine (Baltimore)
June 2024
Department of Medical Laboratory Science, Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, China.
To investigate the expression of Inhibin B between various clinical stages, Chinese medicine dialectic typing, and in nasopharyngeal carcinoma (NPC) tissues and serum, and to evaluate the potential of Inhibin B as a new biomarker for NPC. Paraffin specimens of pathologically confirmed NPC tissues and paracancerous tissues were retrospectively collected, and the expression of Inhibin α (INHA) and Inhibin βB (INHBB) was detected by SP method, and their relationship with clinicopathological indexes was analyzed; in addition, patients with NPC who had received radiotherapy were included as the study subjects, and Epstein-Barr virus DNA (EBV-DNA), INHA, and INHBB in patients were detected by using the fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and chemiluminescent immuno-sandwiching method, respectively. EBV-DNA, EBV-viral capsid antigen-immunoglobulin A (VCA IgA), INHA, and INHBB were detected in the patients, respectively, and their relationships with traditional Chinese medicine (TCM) patterns were also analyzed.
View Article and Find Full Text PDFInt J Mol Sci
April 2023
Isotope Research Lab, College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
Inhibins suppress the FSH production in pituitary gonadotrope cells by robustly antagonizing activin signaling by competitively binding to activin type II receptors (ACTR II). The binding of inhibin A to ACTR II requires the presence of its co-receptor, namely, betaglycan. In humans, the critical binding site for betaglycan to inhibin A was identified on the inhibin α subunit.
View Article and Find Full Text PDFBMC Pregnancy Childbirth
April 2023
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
Background: This study aimed to evaluate the predictive power of a model combining maternal risk factors and the Quadruple screen test for late-onset preeclampsia (PE).
Methods: All pregnant women that received the Quadruple test for Down syndrome at 15-20 weeks' gestation were recruited. Maternal serum α-fetoprotein, β-human chorionic gonadotropin, unconjugated estriol, and inhibin A were measured as multiples of the median.
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