Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed. Special attention is paid to diphasmids lambda ZAP, lambda SK12, lambda SK15, that combine features of lambda and M13 phages and of plasmids. The convenience and difficulties of "jumping" libraries for physical mapping of chromosomes are briefly considered.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A butterfly-shaped acceptor with rigid skeleton and unique assembly enables both efficient organic photovoltaics and high-speed organic photodetectors.
Natl Sci Rev
January 2025
State Key Laboratory of Elemento-Organic Chemistry, Frontiers Science Center for New Organic Matter, The Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, Renewable Energy Conversion and Storage Center (RECAST), Tianjin Key Laboratory of Functional Polymer Materials, Nankai University, Tianjin 300071, China.
It remains challenging to design efficient bifunctional semiconductor materials in organic photovoltaic and photodetector devices. Here, we report a butterfly-shaped molecule, named WD-6, which exhibits low energy disorder and small reorganization energy due to its enhanced molecular rigidity and unique assembly with strong intermolecular interaction. The binary photovoltaic device based on PM6:WD-6 achieved an efficiency of 18.
View Article and Find Full Text PDFThe status quo and influencing factors of breastfeeding social support in China based on LASSO regression model.
PeerJ
January 2025
School of Nursing, Chengde Medical University, Chengde, Hebei Province, China.
Background: In China, the rate of exclusive breastfeeding at 6 months is only 29.2%, well below the global breastfeeding collective target of at least 50% by 2025. This study explores the status quo of breastfeeding social support among and analyses its influencing factors, in order to provide a basis for improving breastfeeding rate in China.
View Article and Find Full Text PDFLongitudinal performance trajectories of young female sprint runners: a new tool to predict performance progression.
Front Sports Act Living
December 2024
Department of Elite Sport, Swiss Federal Institute of Sport Magglingen, Magglingen, Switzerland.
Background: Longitudinal performance tracking in sports science is crucial for accurate talent identification and prognostic prediction of future performance. However, traditional methods often struggle with the complexities of unbalanced datasets and inconsistent repeated measures.
Purpose: This study aimed to analyze the longitudinal performance development of female 60 m sprint runners using linear mixed effects models (LMM).
Spontaneous Resolution of the Fe(CO) Anion and Inclusion of Chiral Guest Molecules in BEDT-TTF Radical-Cation Salts.
Inorg Chem
January 2025
School of Science and Technology, Nottingham Trent University, Nottingham, Clifton Lane NG11 8NS, U.K.
We report the synthesis of three radical-cation salts of BEDT-TTF from racemic tris(oxalato)ferrate by electrocrystallization in the presence of chiral molecules. In the presence of enantiopure l-(+)-tartaric acid, we observe spontaneous resolution of the labile tris(oxalato)ferrate anion to produce the chiral radical-cation salt α-(BEDT-TTF)[Δ-Fe(CO)].[l-(+)-tartaric acid] which contains only the Δ enantiomer of Fe(CO).
View Article and Find Full Text PDFEnhanced Anti-Interference Photoelectrochemical DNA Bioassay: Grafting a Peptide-Conjugated Hairpin DNA Probe on a COF-Based Photocathode.
ACS Sens
January 2025
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Precise and sensitive analysis of specific DNA in actual human bodily fluids is crucial for the early diagnosis of major diseases and for a deeper understanding of DNA functions. Herein, by grafting a peptide-conjugated hairpin DNA probe to a covalent organic framework (COF)-based photocathode, a robust anti-interference photoelectrochemical (PEC) DNA bioassay was explored, which could specifically resist potential interference from nonspecific proteins and reducing species. Human immunodeficiency virus (HIV) DNA was used as the target DNA (tDNA) for the PEC DNA bioassay.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!