AI Article Synopsis

  • The study aimed to assess the effectiveness of using PCR on aqueous humor samples to detect and genotype Epstein-Barr Virus (EBV) in patients with viral retinitis.
  • Out of 70 samples from various patient groups, EBV was detected in 37.7% of cases, with genotyping revealing only one subtype (Type 1) present, while CMV was found in 11.1% of samples.
  • This research marks the first instance of conducting EBV genotyping on intraocular samples, suggesting that PCR can serve as a valuable tool for clinical management and epidemiological studies.

Article Abstract

Purpose: To evaluate the diagnostic value of PCR on aqueous humour for detection and genotyping of Epstein Bar Virus in patients with viral retinitis.

Methods: 70 AH samples were collected from 20 HIV positive patients with clinically suspected viral retinitis and 25 patients with serpignous choroiditis and 25 AH from patients undergoing cataract surgery. PCR was performed to screen HHV-1 to HHV-5, Mtb and Toxoplasma gondii. Genotype prevalence was confirmed by phylogenetic analysis targetig EBV.

Results: EBV was detected in 17 (37.7%) samples. Genotyping to subtype EBV, revealed the circulation of only one subtype (Type 1). PCR results for other infective agents were negative except for the presence of CMV in 5 (11.1%) AH.

Conclusion: The application of PCR to detect genotypes can be used as an epidemiological tool for clinical management. To our knowledge this is the first report on genotyping of EBV performed on intra ocular samples.

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Source
http://dx.doi.org/10.3109/09273948.2014.968265DOI Listing

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