We use Fourier-Bessel Image Decomposition (FBID) of microscopy images to investigate the size, refractive index and 3-dimensional position of individual colloidal microspheres. With measurements of monodisperse polystyrene and poly(methyl methacrylate) particles we achieve a resolution of 1% in size and 0.2% in refractive index for a single image which is sufficient for accurate in situ characterization of polydisperse colloids. Also the binding of avidin molecules to individual biotinylated polystyrene particles is resolved. Finally, the FBID method offers a straightforward approach to 3-dimensional out-of-focus tracking. Here, the z-position of a freely diffusing particle is calculated by applying the statistics of Brownian motion to its set of Fourier-Bessel coefficients.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OE.22.024635 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Individualised acute normovolaemic haemodilution for non-cardiac surgery with anticipated high-dose red cell transfusion: study protocol for a randomised controlled trial in West China Hospital of Sichuan University.
BMJ Open
January 2025
Department of Anesthesia and Operation Center, West China Hospital of Sichuan University, Chengdu, Sichuan, China
Introduction: Acute normovolaemic haemodilution (ANH) is a perioperative blood management technique involving the removal of whole blood and simultaneous infusion of colloids or crystalloids to achieve haemodilution while maintaining normovolaemia. However, its efficacy in reducing the requirement for perioperative allogeneic blood transfusion remains controversial due to inconsistent findings in the literature. An individualised red cell transfusion strategy, guided by the West China Liu's Score, has demonstrated effectiveness in reducing the need for allogeneic red cell transfusion.
View Article and Find Full Text PDFNanostructural Analysis of Age-Related Changes Affecting Human Dentin.
Calcif Tissue Int
January 2025
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan.
Human dentin performs its function throughout life, even though it is not remodeled like bone. Therefore, dentin must have extreme durability against daily repetitive loading. Elucidating its durability requires a comprehensive understanding of its shape, structure, and anisotropy at various levels of its structure.
View Article and Find Full Text PDFFilsuvez (Birch Triterpenes) Topical Gel.
Skinmed
January 2025
Baylor Scott & White University Hospital, Dallas, TX.
Filsuvez (birch triterpenes) topical gel received approval in 2023 for the treatment of epidermolysis bullosa (EB) in pediatric patients (aged ≥6 months) and adults. It promotes wound healing by modulating inflammation, encouraging new tissue formation, and maintaining the skin barrier. In a randomized, double-blind, controlled, parallel-group, phase III trial (EASE, NCT03068780), 223 patients were randomly assigned to two groups: the first group received treatment with birch triterpenes topical gel (study gel, n = 109), and the second group received treatment with vehicle gel (n = 114).
View Article and Find Full Text PDFAssociation of Mycobacterium tuberculosis aerosolization and HIV coinfection in the index case with T cell responses in household contacts.
Sci Rep
January 2025
Department of Global Health, University of Washington, Seattle, WA, USA.
Exposure to pulmonary tuberculosis (PTB) culminates in heterogeneous outcomes, including variation in Mtb antigen-specific interferon-gamma (IFN-γ) T-cell responses. IFN-γ-independent cytokines, including tumor necrosis factor (TNF) and interleukin (IL-2), offer potential diagnostic improvements and insights into pathogenesis. We hypothesized that ESAT6/CFP10 TNF and IL-2 responses improve Mtb infection detection among exposed household contacts (HHCs) and are associated with index case Mtb aerosolization (i.
View Article and Find Full Text PDFColloidal Nanoparticles Hold Elementary Charge in Nonpolar Solvent.
Nano Lett
January 2025
College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing 210023, China.
The net charge of individual nanoparticles in nonpolar solvents plays a critical role in their intrinsic properties like charge carrier lifetime, electron transport, and interparticle interactions. However, there is a long-standing belief that the oil-dispersed nanoparticles inherently possess no net charge. This work presents an approach for directly quantifying the net charge of individual nanoparticles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!