Evaluation of anti-inflammatory activity of probiotic on carrageenan-induced paw edema in Wistar rats.

Objective: Probiotic strain Lactobacillus sporogenes and Bifidobacteria bifidum were used to assess the anti-inflammatory properties in Carrageenan induced acute inflammatory model.

Methods: Non-encapsulated and encapsulated Probiotic strain of Bifidobacteria bifidum and Lactobacillus sporogenes was given orally. Diclofenac sodium was used as standard drug at a concentration of 150 mg/kg of body weight. Edema was induced with 1% carrageenan to all the groups except group A after half an hour of the oral treatments. Paw thickness was checked at t = 1, 2, 4 and 24 h. Stair climbing score and motility score were assessed at t = 24 h.

Results: Non-encapsulated and encapsulated Probiotic Bifidobacteria and Lactobacillus showed a statistically significant decrease in paw thickness at P < 0.05. The percentage inhibition in paw thickness of non-encapsulated and encapsulated probiotic L. sporogenes and B. bifidum is 37 ± 3% and 43 ± 2% after 24 h of treatment. They both significantly increased stair climbing and motility score.

Conclusion: Probiotic B. bifidum and L. sporogenes significantly decreased the inflammatory reactions induced by carrageenan.