Incubation of Trypanosoma cruzi bloodstream trypomastigotes (Btrys) with C5-deficient blood in the presence of anti-T. cruzi immune mouse serum (IMS) or its IgG fraction resulted in an immediate formation of small clumps in which one could easily see platelets adhered to the parasites. After 4 h of incubation most of the clumps had disappeared and the number of the parasites was considerably reduced. Twenty-four hours later there were only a few remaining parasites. This same sequence of events was also observed when the parasites were incubated with isolated platelets in presence of IMS or its IgG fraction. When the parasites were incubated with plasma in the presence of IMS or its IgG fraction but in the absence of platelets there was strong agglutination of the parasites but no reduction in their number. Incubation of the parasites with platelets and IMS or its IgG fraction in C3-depleted plasma prevented adherence of the platelets to the parasites and their subsequent lysis. It is concluded that platelets are able to induce in-vitro lysis of the Btrys and that this phenomenon is dependent on the platelet' receptor for C3b.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1365-3024.1989.tb00689.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of Recombinant Antibodies and Its Application in Immunomagnetic Separation-Based Rapid Detection of in Aquatic Environments.
J Microbiol Biotechnol
November 2024
Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, P.R. China.
Article Synopsis
- Cholera remains a significant public health threat, especially due to challenges in detecting contamination in drinking water and aquatic environments, mainly because of sample preparation difficulties.
- The study developed immunomagnetic separation methods using recombinant antibodies (rAbs) to efficiently enrich cholera bacteria from water samples, achieving high capture efficiencies within a short time.
- The resulting antibodies showed high specificity and low cross-reactivity with other bacteria, offering promising alternatives for accurate detection in food safety and environmental monitoring applications.
In-situ electrochemically synthesized "artificial" Gly m TI antibody for soybean allergen quantification in complex foods.
Anal Chim Acta
December 2024
Centro de Investigação em Química da Universidade do Porto/ Instituto de Ciências Moleculares (CIQUP/IMS), Faculdade de Ciências da Universidade do Porto, Departamento de Química e Bioquímica Rua do Campo Alegre 687, 4169-007, Porto, Portugal. Electronic address:
Article Synopsis
- Biosensors targeting food allergens, like Gly m TI in soybeans, enhance individual health safety and public health by facilitating compliance with food regulations and reducing allergen-related recalls.
- Recent developments in biosensor technology, like portability and real-time monitoring, broaden their applications in food production, food service, and even home environments, making them essential tools for allergen detection.
- A novel Gly m TI-MIP biosensor was successfully created to identify soybean protein in a variety of complex food matrices, achieving high sensitivity and minimizing cross-reactivity, thus improving food safety protocols for both processed and unprocessed foods.
Comprehensive evaluation of anti-emicizumab antibodies in acquired hemophilia A: a detailed case study and methodological evaluation.
J Thromb Haemost
January 2025
Institute of Experimental Hematology and Transfusion Medicine, University Hospital Bonn, Medical Faculty, University of Bonn, Germany; Center for Rare Diseases Bonn (Zentrum für seltene Erkrankungen der Universität Bonn), University Clinic Bonn, Bonn, Germany. Electronic address:
Background: Acquired hemophilia A (AHA) is a rare and severe bleeding disorder characterized by autoantibodies inhibiting coagulation factor (F)VIII. Current treatment of AHA involves bypassing agents or FVIII replacement therapy, yet their efficacy is limited in cases of high inhibitor titers. Emicizumab, a humanized bispecific monoclonal antibody, has shown promising hemostatic effectiveness in persons with congenital hemophilia A (HA) and AHA, but a minority of patients developed anti-drug antibodies (ADAs), compromising its efficacy.
View Article and Find Full Text PDFγ-Conglutin Immunoreactivity Is Differently Affected by Thermal Treatment and Gastrointestinal Digestion in Lupine Species.
Foods
July 2024
REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Lupine is a legume commonly used in human diet as a functional food due to its high nutritional content and important technological properties. However, its consumption can lead to the manifestation of adverse immunological reactions, posing significant health issues in sensitized/allergic patients. This work aims to investigate the effect of food processing combined with simulated gastrointestinal (GI) digestion on the immunoreactivity of lupine γ-conglutin.
View Article and Find Full Text PDFComparative study on alginate/chitosan microcapsules and Montanide ISA 61 as vaccine adjuvants in mice.
PLoS One
April 2024
Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Selection of adjuvant to be combined with the antigen is an extremely important point for formulating effective vaccines. The aim of this study was to evaluate reactogenicity, levels of IgM, IgG and subclasses (IgG1, IgG2b and IgG3), and protection elicited by vaccine formulations with association of chitosan coated alginate or Montanide ISA 61 with γ-irradiated Brucella ovis. The alginate/chitosan biopolymers as well as the Montanide ISA 61 emulsion elicited intense and long-lasting local response, especially when associated with the antigen.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!