An improved surface passivation method for single-molecule studies.

Boyang Hua Kyu Young Han Ruobo Zhou Hajin Kim Xinghua Shi Sanjaya C Abeysirigunawardena Ankur Jain Digvijay Singh Vasudha Aggarwal Sarah A Woodson Taekjip Ha

Nat Methods

1] Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [4] Howard Hughes Medical Institute, Urbana, Illinois, USA. [5] Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Published: December 2014

We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.

Download full-text PDF	Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245390	PMC
http://dx.doi.org/10.1038/nmeth.3143	DOI Listing

Publication Analysis

Top Keywords

surface passivation

passivation method

single-molecule studies

improved surface

method single-molecule

studies report

report surface

method based

based dichlorodimethylsilane

dichlorodimethylsilane dds-tween-20

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!