[MALDI-TOF mass-spectrometric analysis of cell proteins during identification of leptospira genus members].

Aim: Development of methodological approaches for identification of leptospira by using MALDI-TOF direct protein profiling technology.

Materials And Methods: Analysis of cell proteins of 34 leptospira strains was carried out in Microflex LT by using "MALDI Biotyper 3.0 for identification and classification of microorganisms" program.

Results: 19 reference spectra of reference leptospira strains from 7 species were generated and imported into MALDI Biotyper 3.0 database. Identification of 6 strains with undetermined taxonomic position was carried out.

Conclusion: The approved method allows determination of leptospira species with accuracy that depends on their adaptation to nutrient media, preparation approach and sample storage conditions for mass-spectrometry.