AI Article Synopsis
- The study aimed to evaluate how well CM-Dil and GFP label human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) for tracking purposes after transplantation into guinea pigs with nasal mucosa radiation injury.
- Both labeling methods showed high efficiency in vitro, with CM-Dil achieving a 95% labeling success rate and GFP reaching 90% at the first cell passage.
- While both labels did not affect the cells' ability to differentiate into multiple lineages, GFP labeling negatively impacted hUC-MSC proliferation due to virus toxicity.
Article Abstract
To investigate the effectiveness of chloromethyl-dialkylcarbocyanine (CM-Dil) and green florescent protein (GFP) for tracking transplanted stem human umbilical cord-derived mesenchymal stem cells (hUC-MSCs), hUC-MSCs were labeled with CM-Dil or GFP and transplanted into guinea pigs with nasal mucosa radiation injury. In vitro and in vivo labeling efficiency was investigated by flow cytometry and fluorescence microscopy. Proliferation and multi-lineage differentiation potential of labeled hUC-MSCs were assessed via cell quantification and specific staining. In vitro CM-Dil and GFP labeling efficiency was 95 ± 12.2 and 90 ± 8 % at first passage, respectively. Labeled hUC-MSCs were detected by fluorescence microscopy 10 days (CM-Dil) or 20 days (GFP) after transplantation. Neither type of cell labeling affected the multi-differentiation potential but GFP labeling inhibited hUC-MSC proliferation due to virus toxicity. hUC-MSCs can be labeled with either CM-Dil or GFP with high efficiency without impacting multi-differentiation potential.
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| http://dx.doi.org/10.1007/s10529-014-1692-1 | DOI Listing |
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Article Synopsis
- The study aimed to evaluate how well CM-Dil and GFP label human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) for tracking purposes after transplantation into guinea pigs with nasal mucosa radiation injury.
- Both labeling methods showed high efficiency in vitro, with CM-Dil achieving a 95% labeling success rate and GFP reaching 90% at the first cell passage.
- While both labels did not affect the cells' ability to differentiate into multiple lineages, GFP labeling negatively impacted hUC-MSC proliferation due to virus toxicity.
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