AI Article Synopsis
- Toxocariasis is a disease caused by a parasite, and measuring IgM levels can make it hard to tell if the infection is new or ongoing.
- This study aimed to use a method called indirect ELISA, combined with urea treatment, to distinguish between acute and chronic cases of Toxocariasis in mice.
- Results showed that IgG avidity was low (7.25-27.5%) in the first 15 days of infection, indicating an acute infection, but increased (31.4-58%) after 60 days, suggesting a shift to chronic infection.
Article Abstract
Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.
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| http://dx.doi.org/10.1590/s1984-29612014060 | DOI Listing |
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