To improve the hydrolysis and acidification of activated sludge, two new ways, which were adding fermented sludge or sterilized fermented sludge to batch-mode activated sludge alkaline fermentation system, were investigated in this study. Temperature effect was also studied (10 degrees C and 35 degrees C). Result showed that mesophilic condition greatly enhanced sludge hydrolysis and acidification, which resulted in an obvious volatile fatty acids (VFAs) accumulation in the system. These two new ways could improve both sludge hydrolysis and acidification. The hydrolysis and acidification efficiencies of adding sterilized fermented sludge system were 2 and 1.5 times higher than those of adding fermented sludge system, respectively. In addition, VFAs content was nearly constant in a long time in sterilized fermented sludge addition system while it greatly consumed in fermented sludge addition system. The reason for this enhancement of two new ways were different, fermented sludge addition increased the amount of hydrolytic enzyme and acid-forming bacteria, while sterilized fermented sludge addition increased the amount of degradable substances, and the result also demonstrated that strengthen hydrolysis was more important than acidification. Therefore, mesophilic condition together with sterilized fermented sludge addition was a more effective way to enhance VFAs accumulation in alkaline sludge fermentation process.
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Bioresour Technol
January 2025
Department of Environmental Technologies, Faculty of Marine and Environmental Sciences, IVAGRO-Wine and Agrifood Research Institute, University of Cadiz, 11510 Puerto Real, Cadiz, Spain.
This study investigates the effects of ozone pre-treatment on two types of organic wastes: secondary sludge (SS) and wine vinasse (WV). Ozone pre-treatment of SS, a semi-solid waste, significantly increased the Dissolved Organic Carbon (DOC) and Total Volatile Fatty Acids (TVFAs) through hydrolysis. Conversely, ozone pre-treatment of WV, a liquid organic waste, reduced the availability of soluble biodegradable substrates and decreased the concentration of carboxylic acids with carbon chain length higher than 4.
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January 2025
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, 210044, China.
With the aim of exploring the association between microbial response and volatile fatty acids (VFAs) production in sludge and chicken manure co-fermentation with total solids (TS) controlled, four fermentation experimental groups (TS = 20, 40, 60, and 80 g/L) were established in this study. The results demonstrated that the yield of VFAs reached the peak (530.08 mg COD/g VSS) at the 40 g-TS group.
View Article and Find Full Text PDFBioresour Bioprocess
January 2025
Biotechnology Processes Unit, IMDEA Energy, Avda. Ramón de la Sagra 3, Móstoles, Madrid, 28935, Spain.
This research investigated the acidogenic fermentation (AF) of sugar cane molasses in an up-flow anaerobic sludge blanket (UASB) reactor for the production of carboxylates. The first step was to assess the optimum process temperature (25, 35 or 55 ºC) using two different granular inocula, one from a brewery company (BGS) and other from a paper plant company (PGS). These experiments determined that the most suitable temperature for carboxylates production was 25 ºC, obtaining higher bioconversions (27.
View Article and Find Full Text PDFMembranes (Basel)
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DVGW-Research Center at the Engler-Bunte-Institute, Water Chemistry and Water Technology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 9, 76131 Karlsruhe, Germany.
Short-chain fatty acids (SCFAs) are valuable metabolic intermediates that are produced during dark fermentation of sludge, which, when capitalized on, can be used as chemical precursors for biotechnological applications. However, high concentrations of solids with SCFAs in hydrolyzed sludge can be highly detrimental to downstream recovery processes. This pilot-scale study addresses this limitation and explores the recovery of SCFAs from primary sludge into a particle-free permeate through a combination of chamber filter-press (material: polyester; mesh size: 100 µm) and cross-flow microfiltration (material: α-AlO; pore size: 0.
View Article and Find Full Text PDFWater Res
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Department of Civil and Environmental Engineering, University of Florence, Via di S. Marta, 3, 50139, Firenze, Italy.
The performance of Upflow Anaerobic Sludge Blanket (UASB) bioreactors treating sulfate (SO) -rich effluents depends on multiple factors, including microbial interactions and operational conditions. The high complexity of these systems necessitates the use of mathematical modelling tools to better understand the process and predict the long-term impacts of various operational variables. In this work, a mathematical model describing the long-term operation of a sulfate-fed 2.
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