Detection of cyclic di-AMP using a competitive ELISA with a unique pneumococcal cyclic di-AMP binding protein.

J Microbiol Methods

Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA. Electronic address:

Published: December 2014

Cyclic di-AMP (c-di-AMP) is a recently recognized bacterial signaling molecule. In this study, a competitive enzyme-linked immunosorbent assay (ELISA) for the quantification of c-di-AMP was developed using a novel pneumococcal c-di-AMP binding protein (CabP). With this method, c-di-AMP concentrations in biological samples can be quickly and accurately quantified.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252578PMC
http://dx.doi.org/10.1016/j.mimet.2014.08.026DOI Listing

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