AI Article Synopsis
- The text discusses how combining electron microscopy (EM), X-ray crystallography, and molecular dynamics (MD) simulations can enhance our understanding of complex macromolecular structures and their dynamics.
- It highlights two specific examples: the ribosome and the Arp2/3-actin complex, where these techniques were used to uncover functional dynamics.
- The third example focuses on the GPCR:G protein complex, showcasing how these methods can provide insights into transmembrane signaling, underscoring the advancements in integrative structural biology.
Article Abstract
Three vignettes exemplify the potential of combining EM and X-ray crystallographic data with molecular dynamics (MD) simulation to explore the architecture, dynamics and functional properties of multicomponent, macromolecular complexes. The first two describe how EM and X-ray crystallography were used to solve structures of the ribosome and the Arp2/3-actin complex, which enabled MD simulations that elucidated functional dynamics. The third describes how EM, X-ray crystallography, and microsecond MD simulations of a GPCR:G protein complex were used to explore transmembrane signaling by the β-adrenergic receptor. Recent technical advancements in EM, X-ray crystallography and computational simulation create unprecedented synergies for integrative structural biology to reveal new insights into heretofore intractable biological systems.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387792 | PMC |
| http://dx.doi.org/10.1016/j.sbi.2014.08.006 | DOI Listing |
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