AI Article Synopsis
Article Abstract
In this study complex I was immobilized in a biomimetic environment on a gold layer deposited on an ATR-crystal in order to functionally probe the enzyme against substrates and inhibitors via surface-enhanced IR absorption spectroscopy (SEIRAS) and cyclic voltammetry (CV). To achieve this immobilization, two methods based on the generation of a high affinity self-assembled monolayer (SAM) were probed. The first made use of the affinity of Ni-NTA toward a hexahistidine tag that was genetically engineered onto complex I and the second exploited the affinity of the enzyme toward its natural substrate NADH. Experiments were also performed with complex I reconstituted in lipids. Both approaches have been found to be successful, and electrochemically induced IR difference spectra of complex I were obtained.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi500955a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evolution of branched peptides as novel biomaterials.
J Mater Chem B
January 2025
University of Bath, Claverton Down, Bath, BA2 7AY, UK.
Branched peptide-based materials draw inspiration from dendritic structures to emulate the complex architecture of native tissues, aiming to enhance the performance of biomaterials in medical applications. These innovative materials benefit from several key features: they exhibit slower degradation rates, greater stiffness, and the ability to self-assemble. These properties are crucial for maintaining the structural integrity and functionality of the materials over time.
View Article and Find Full Text PDFEstablishment of a Low-Cost and Efficient In Vitro Model for Cultivating Intestinal Microbiota.
J Agric Food Chem
January 2025
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
The Simulator of Human Intestinal Microbial Ecosystem (SHIME) has hindered widespread adoption due to its high cost. This study founded biomimetic multilink fermentation equipment (BMLFE), priced at half or even lower than SHIME. It was improved based on multilink fermentation equipment (MLFE) by modifying materials, peristaltic pumps, fermentation time, and dietary habits while calculating transfer time and volumes and conducted anaerobic fermentation for 15 days followed by monitoring changes in intestinal microbial composition and short-chain fatty acids (SCFAs).
View Article and Find Full Text PDFA Fused Membrane-Camouflaged Biomimetic Nanosystem for Dual-Targeted Therapy of Septic Arthritis.
Small
January 2025
Sports Medicine Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Due to the inherent aseptic and enclosed characteristics of joint cavity, septic arthritis (SA) almost inevitably leads to intractable infections and rapidly progressing complex pathological environments. Presently, SA faces not only the deficient effectiveness of the gold-standard systemic antibiotic therapy but also the scarcity of effective localized targeted approaches and standardized animal models. Herein, an ingenious multifunctional nanosystem is designed, which involves the methylation of hyaluronic acid (HA), copolymerization with DEGDA, loading with vancomycin (VAN), and then coating with fused macrophage-platelet membrane (denoted as FM@HA@VAN).
View Article and Find Full Text PDFBoosting Thermal Generation in Cation-Exchanged Transparent MXene Composite Films with Hierarchical Wrinkled Structure.
ACS Appl Mater Interfaces
January 2025
School of Integrated Circuits and Electronics, Beijing Institute of Technology, Beijing 100081, P. R. China.
Efficient thermal generation from solar/electric energy in transparent films remains challenging due to the limited toolbox of high-performance thermal generation materials and methods for microstructure engineering. Here, we proposed a two-step strategy to introduce hierarchical wrinkles to the MXene composite films with high transparency, leading to upgraded photo/electrothermal conversion efficiency. Specifically, the thin film contains protic acid-treated MXene layers assembled with Ag nanowires (H-MXene/Ag NWs).
View Article and Find Full Text PDFBiomimetic Confined Assembly of Plasmonic CuS from Electronic Waste for Rapid Photothermal Disinfection.
ACS Nano
January 2025
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Photothermal disinfection (PTD) offers promising potential for water purification due to its sustainable and broad-spectrum bactericidal properties, although it is hindered by slow charge separation in photosensitizers. Herein, we present a plasma-mediated PTD technique utilizing an efficient localized heating effect induced by incident light at specific wavelengths for rapid bacterial inactivation. A metallic CuS photosensitizer, derived from electronic waste through a biomimetic transmembrane confined-assembled strategy, facilitates collective and coherent oscillation of free electrons around Cu atoms in the near-infrared range.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!