AI Article Synopsis
- Rats are commonly used in biomedical research, and the recent development of true pluripotent rat embryonic stem cell lines opens up new possibilities for genetic engineering in scientific studies.* -
- Improvements in the stability of rat embryonic stem cell cultures will enhance their application and effectiveness in research.* -
- The text presents a straightforward protocol for maintaining germ line competent rat ES cells and suggests using small molecule inhibitors to enhance self-renewal and assess differentiation potential.*
Article Abstract
The rat is one of the most commonly used laboratory animals in biomedical research and the recent isolation of genuine pluripotent rat embryonic stem (ES) cell lines has provided new opportunities for applying contemporary genetic engineering techniques to the rat and enhancing the use of this rodent in scientific research. Technical refinements that improve the stability of the rat ES cell cultures will undoubtedly further strengthen and broaden the use of these stem cells in biomedical research. Here, we describe a relatively simple and robust protocol that supports the propagation of germ line competent rat ES cells, and outline how tuning stem cell signaling using small molecule inhibitors can be used to both stabilize self-renewal of rat ES cell cultures and aid evaluation of their differentiation potential in vitro.
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| http://dx.doi.org/10.1007/7651_2014_121 | DOI Listing |
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