AI Article Synopsis
- In situ hybridization (ISH) technique enables researchers to see specific RNA sequences in embryos, which helps to study gene expression during development or after experimental changes.
- The use of short DNA probes with locked nucleic acid nucleotides (LNAs) enhances the detection of specific RNA variations, opening up new diagnostic options.
- The described methods focus on using 14-24 nt LNA-containing probes to identify moderately to highly expressed RNAs in chick embryos within the first five days of development, and the protocol can be adjusted for embryos from other vertebrate species.
Article Abstract
In situ hybridization (ISH) in embryos allows the visualization of specific RNAs as a readout of gene expression during normal development or after experimental manipulations. ISH using short DNA probes containing locked nucleic acid nucleotides (LNAs) holds the additional advantage of allowing the detection of specific RNA splice variants or of closely related family members that differ in only short regions, creating new diagnostic and detection opportunities. Here we describe methods for using short (14-24 nt) DNA probes containing LNA nucleotides to detect moderately to highly expressed RNAs in whole chick embryos during the first 5 days of embryonic development. The protocol is easily adaptable for use with embryos of other vertebrate species.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148130 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-1459-3_6 | DOI Listing |
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