AI Article Synopsis

  • - The study examines how three proteins (lysozyme, ovalbumin, ovotransferrin) interact with synthetic smectite particles, using atomic force microscopy and quartz crystal microbalance to analyze these interactions in water at varying pH levels.
  • - Key findings indicate that the binding of proteins to the smectite is mainly governed by electrostatic interactions, with protein behavior changing at different pH levels—high pH leads to changes in lysozyme orientation and instability of ovalbumin, while low pH causes desorption of lysozyme and swelling of ovalbumin.
  • - Ovotransferrin showed the least stability in its complex with smectite, consistently desorbing across pH

Article Abstract

We report on adsorption of lysozyme (LYS), ovalbumin (OVA), or ovotransferrin (OVT) on particles of a synthetic smectite (synthetic layered aluminosilicate). In our approach we used atomic force microscopy (AFM) and quartz crystal microbalance (QCM) to study the protein-smectite systems in water solutions at pH ranging from 4 to 9. The AFM provided insights into the adhesion forces of protein molecules to the smectite particles, while the QCM measurements yielded information about the amounts of the adsorbed proteins, changes in their structure, and conditions of desorption. The binding of the proteins to the smectite surface was driven mainly by electrostatic interactions, and hence properties of the adsorbed layers were controlled by pH. At high pH values a change in orientation of the adsorbed LYS molecules and a collapse or desorption of OVA layer were observed. Lowering pH to the value ≤ 4 caused LYS to desorb and swelling the adsorbed OVA. The stability of OVT-smectite complexes was found the lowest. OVT revealed a tendency to desorb from the smectite surface at all investigated pH. The minimum desorption rate was observed at pH close to the isoelectric point of the protein, which suggests that nonspecific interactions between OVT and smectite particles significantly contribute to the stability of these complexes.

Download full-text PDF

Source
http://dx.doi.org/10.1021/la502840sDOI Listing

Publication Analysis

Top Keywords

smectite particles
12
proteins smectite
8
smectite surface
8
smectite
6
adsorption aggregation
4
desorption
4
aggregation desorption
4
desorption proteins
4
particles
4
particles report
4

Similar Publications

Redox Properties of Structural Fe in Clay Minerals: 4. Reinterpreting Redox Curves by Accounting for Electron Transfer and Structural Rearrangement Kinetics.

Environ Sci Technol

November 2024

Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, State College, Pennsylvania 16802, United States.

Iron-bearing smectite clay minerals can act as electron sources and sinks in the environment. Previous studies using mediated electrochemical analyses to determine the reduction potential () values of smectites observed that the relationship between the structural Fe/Fe ratio in the smectite and varied based on the redox history of the smectite. We hypothesize that this behavior, referred to as redox hysteresis, results from the smectite particles not equilibrating with the applied over the course of the experiment (∼30 min).

View Article and Find Full Text PDF

A synthetic and transparent clay removes Microcystis aeruginosa efficiently.

Harmful Algae

August 2024

University of Minnesota, Minneapolis, MN, USA; Department of Civil, Environmental, and Geo Engineering, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address:

Clay-algae flocculation is a promising method to remove harmful algal blooms (HABs) in aquatic ecosystems. Many HAB-generating species, such as Microcystis aeruginosa (M. aeruginosa), a common species in lakes, produce toxins and harm the environment, human health, and the economy.

View Article and Find Full Text PDF

The global rise in obesity necessitates innovative weight loss strategies. Naturally occurring smectite clays, such as montmorillonite (MMT), offer promise due to their unique properties that interfere with free fatty acid (FFA) liberation, reducing systemic uptake. However, the mechanisms of MMT-FFA interactions and their implications for weight management are undefined.

View Article and Find Full Text PDF

Influence of Sol-Gel State in Smectite Aqueous Dispersions on Drying Patterns of Droplets.

Materials (Basel)

June 2024

Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

The sol-gel state of smectite clay dispersions varies with the volume fraction of clay and electrolyte concentration. In this study, it was elucidated that the drying patterns of droplets from four types of smectite clay dispersions vary according to their sol-gel states. Droplets in the sol state exhibited a ring-shaped pattern, while those in the gel state showed a bump-shaped pattern.

View Article and Find Full Text PDF

The occurrence and genesis of grain-coating chlorite were investigated in order to evaluate the impact of grain-coating chlorite on preserving porosity in the deep-buried Triassic Karamay volcaniclastic sandstones based on thin sections, scanning electron microscopy, and an electron probe. Grain-coating chlorite was formed during the eogenesis, originating from the precursor of smectite through the solid-state transformation (SST) mechanism. The hydration and dissolution of unstable, intermediately basic volcanic rock fragments provided essential Fe and Mg ions for the formation of grain-coating chlorite.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: