Objectives: We tested if modulation of cytosolic K(+) levels by ouabain, an inhibitor of Na(+) /K(+) -ATPase, exerts cytoprotection against distinct stressful stimuli in human leukemic cells.
Methods: The cytosolic K(+) , Na(+) or Ca(2+) levels and the cytotoxicity were evaluated by flow cytometry.
Key Findings: Various cytotoxic chemicals and ultraviolet irradiation induced cell death and increased intracellular concentrations of K(+) , Na(+) or Ca(2+) . Ouabain reduced the cytotoxicity and the elevation of cytosolic levels of K(+) but not those of Na(+) or Ca(2+) .
Conclusions: Our data thus suggest that elevated cytosolic K(+) levels are associated with the cytotoxicity in response to distinct stressful stimuli and that ouabain exerts cytoprotection most probably by regulating intracellular K(+) levels.
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