With the aim of characterizing the active ingredients lupenone and β-sitosterol in Rhizoma Musae samples a reversed-phase HPLC method for the separation of these two compounds in Rhizoma Musae samples was developed (regression coefficient>0.9996). The method was further applied to quantify lupenone and β-sitosterol content in Rhizoma Musae samples cultured in different growth environments. Different variables such as geographical location, growth stage, and harvest time, demonstrated differential effects on lupenone and β-sitosterol levels. Moreover, we determined the optimum conditions for cultivation and harvesting of Rhizoma Musae herbs. Lupenone administration caused a significant reduction in fasting blood glucose (FBG) levels in diabetic rats at doses of 1.78, 5.33, and 16.00 mg·kg⁻¹·day⁻¹ for 14 days, the glycated hemoglobin (HbA1c) levels of diabetic rats also significantly reduced at doses of 5.33, and 16.00 mg·kg⁻¹·day⁻¹, indicating a robust antidiabetic activity. To our knowledge, this is the first report of an optimized HPLC method successfully applied to quantify lupenone and β-sitosterol, and its applicability in optimizing Rhizoma Musae growth. Animal experiments also showed for the first time that lupenone from Rhizoma Musae has anti-diabetic activity.
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http://dx.doi.org/10.3390/molecules190914114 | DOI Listing |
Front Pharmacol
January 2025
GuiZhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China.
Objective: This study aimed to identify the potential active compounds in Rhizoma Musae decoction and understand their mechanisms of action in osteoarthritis treatment.
Methods: UHPLC-Q-Exactive-MS/MS technology was used for an in-depth analysis of the chemical compounds present in Rhizoma Musae decoction. A network analysis approach was used to construct a comprehensive network of compounds, targets, and pathways, which provided insights into the molecular mechanisms of Rhizoma Musae decoction in osteoarthritis treatment.
PLoS One
January 2025
GuiZhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China.
Background: Fracture disrupts the integrity and continuity of the bone, leading to symptoms such as pain, tenderness, swelling, and bruising. Rhizoma Musae is a medicinal material frequently utilized in the Miao ethnic region of Guizhou Province, China. However, its specific mechanism of action in treating fractures remains unknown.
View Article and Find Full Text PDFMol Divers
February 2020
Experiment Center, Guiyang University of Chinese Medicine, 50, Nanming District, Guiyang, 550002, Guizhou Province, People's Republic of China.
The dried rhizome of Musa basjoo Sieb. et Zucc. is Rhizoma Musae.
View Article and Find Full Text PDFJ Chromatogr Sci
June 2016
Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang City, Guiyang, Guizhou Province 550002, PR China
Rhizoma Musae has been used for centuries in Miao medicine practice in China, and it usually uses for treating diabetes and bruises. In this study, lupenone and β-sitosterol in Rhizoma Musae were separated by reversed-phase ultra-performance liquid chromatography (RP-UPLC) and simultaneously detected by a diode array detector (DAD) and an evaporative light scattering detector (ELSD) using methanol and 0.1% aqueous acetic acid (100 : 4, v/v) as a mobile phase in 20 min.
View Article and Find Full Text PDFMolecules
September 2014
Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China.
With the aim of characterizing the active ingredients lupenone and β-sitosterol in Rhizoma Musae samples a reversed-phase HPLC method for the separation of these two compounds in Rhizoma Musae samples was developed (regression coefficient>0.9996). The method was further applied to quantify lupenone and β-sitosterol content in Rhizoma Musae samples cultured in different growth environments.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!