AI Article Synopsis
- The text describes a novel method for engineering cell surfaces using liposome fusion delivery, allowing for the creation of a dual-function system that can be remotely controlled to assemble and disassemble microtissues.
- This approach involves modifying cell surface properties with chemoselective functional groups, which provides precise control over how cells interact through new techniques like flow cytometry and lipid pull-down mass spectrometry.
- The technique enables dynamic manipulation of complex tissue structures, facilitating applications in areas like stem cell differentiation, drug delivery, and regenerative medicine while advancing our understanding of cellular communication.
Article Abstract
We report a general cell surface molecular engineering strategy via liposome fusion delivery to create a dual photo-active and bio-orthogonal cell surface for remote controlled spatial and temporal manipulation of microtissue assembly and disassembly. Cell surface tailoring of chemoselective functional groups was achieved by a liposome fusion delivery method and quantified by flow cytometry and characterized by a new cell surface lipid pull down mass spectrometry strategy. Dynamic co-culture spheroid tissue assembly in solution and co-culture tissue multilayer assembly on materials was demonstrated by an intercellular photo-oxime ligation that could be remotely cleaved and disassembled on demand. Spatial and temporal control of microtissue structures containing multiple cell types was demonstrated by the generation of patterned multilayers for controlling stem cell differentiation. Remote control of cell interactions via cell surface engineering that allows for real-time manipulation of tissue dynamics may provide tools with the scope to answer fundamental questions of cell communication and initiate new biotechnologies ranging from imaging probes to drug delivery vehicles to regenerative medicine, inexpensive bioreactor technology and tissue engineering therapies.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159631 | PMC |
| http://dx.doi.org/10.1038/srep06313 | DOI Listing |
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