Premise Of The Study: Ten microsatellite markers were developed and characterized in a gynodioecious summer-deciduous shrub, Daphne jezoensis, to facilitate studies of the evolution of gynodioecy in the species. •
Methods And Results: We used a next-generation sequencing approach with the Ion Personal Genome Machine (PGM) system to identify and develop microsatellite markers with perfect di- and trinucleotide repeats. These markers were tested with 47 samples from two natural populations. The mean observed and expected heterozygosities per population ranged from 0.40 to 0.46 and 0.60 to 0.66, respectively. •
Conclusions: The developed markers will be useful to study the mating system, gene flow, and population genetic structure of D. jezoensis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103110 | PMC |
| http://dx.doi.org/10.3732/apps.1400001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Development of DNA molecular identity cards for germplasm of Murraya paniculata based on SSR markers].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700, China.
To promote the conservation and utilization of the germplasm resources and provide a basis for the breeding of new varieties of Murraya paniculata, this study analyzed the genetic diversity of the germplasm resources and developed the molecular identity(ID) card of M. paniculata. Multiple fluorescence PCR-capillary electrophoresis was performed for 65 germplasm accessions of M.
View Article and Find Full Text PDF[Establishment and application of chloroplast genome database with the largest number of species in world].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
The chloroplast genome is an important tool for studying plant classification, evolution, and the heterologous production of secondary metabolites and protein drugs. With advancements in sequencing technology and reductions in sequencing costs, chloroplast genome data have rapidly accumulated. However, existing chloroplast genome databases suffer from issues such as incomplete data, inadequate management, and inconsistent, inaccurate information, posing significant challenges for the development and utilization of the chloroplast genome.
View Article and Find Full Text PDFATXN2 polyglutamine intermediate repeats length expansions in Malaysian patients with amyotrophic lateral sclerosis (ALS).
Neurogenetics
January 2025
Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
Intermediate CAG repeats from 29 to 33 in the ATXN2 gene contributes to the risk of amyotrophic lateral sclerosis (ALS) in European and Asian populations. In this study, 148 ALS patients of multiethnic descent: Chinese (56.1%), Malay (24.
View Article and Find Full Text PDFReproductive strategies in loggerhead sea turtle : polyandry and polygyny in a Southwest Atlantic rookery.
PeerJ
January 2025
Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, Laboratório de Genética e Evolução Molecular, Vitória, Espírito Santo, Brazil.
Sea turtles are highly migratory and predominantly inhabit oceanic environments, which poses significant challenges to the study of their life cycles. Research has traditionally focused on nesting females, utilizing nest counts and mark-recapture methods, while male behavior remains understudied. To address this gap, previous studies have analyzed the genotypes of females and hatchlings to indirectly infer male genotypes and evaluate the extent of multiple paternity within populations.
View Article and Find Full Text PDFChloroplast Genome Diversity and Molecular Evolution in Hypericaceae: New Insights from Three Species.
Int J Mol Sci
January 2025
School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
The Hypericaceae family, comprising nine genera and over seven hundred species, includes plants traditionally used for medicinal purposes. In this study, we performed high-throughput sequencing on three species: , , and , and conducted comparative genomic analyses with related species. The chloroplast genome sizes were 152,654 bp, 122,570 bp, and 137,652 bp, respectively, with an average GC content of 37.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!