Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae).

Premise Of The Study: Microsatellite markers were isolated and characterized in Shorea platyclados (Dipterocarpaceae) for DNA profiling and genetic diversity assessment of this tropical timber species. •

Methods And Results: Fifteen polymorphic microsatellite loci were developed and characterized in S. platyclados using a genomic library enriched for dinucleotide (CT) repeats. The primers amplified dinucleotide repeats with 3-14 alleles per locus across four natural populations. The observed and expected heterozygosities ranged from 0.292 to 1.000 and from 0.301 to 0.894, respectively. No significant deviation from Hardy-Weinberg equilibrium was detected in the 15 loci. Four loci pairs displayed linkage disequilibrium. •

Conclusions: These highly polymorphic markers are adequate for DNA profiling and studies of population genetics in S. platyclados.