Objective: In order to detect and monitor preservative component in cosmetic, the method for determination of five kinds of restricted substances benzoic acid, chloroxylenol, o-phenyl phenol and p-chloro-m-cresol chlorhexidine in cosmetics by HPLC was carried out.
Methods: 10 kinds of commercial cosmetics as test samples, using methanol as the extracting solution, saturated sodium chloride solution as precipitant, and Agilent Eclipse XDB-C18 (250 mm x 4.6 mm, 5 microm) as analytical column methanol -0.1 nol/L sodium dihydrogen phosphate solution (pH 3.0) was as mobile phase eluting at a flow rate 1.0 ml/min, the detective wavelength was 285 nm.
Results: The method for determination of chlorhexidine and other four kinds of restricted substances had good linear relationship, the linear correlation coefficients were greater than 0.9990, the limit of detection ( LOD) of 0.8 - 2.2 ng, the average recoveries were between 95.0% -104.0%.
Conclusion: The method is accurate and reliable, and it is a useful supplement for "Hygienic Standard for Cosmetics".
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